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タイトル: Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method
著者: MIURA, Yoshiaki
SHIOMI, Akira
SHIRAISHI, Junya
MAKITA, Kohei
ASAKAWA, Mitsuhiko
KITAZAWA, Takio
HIRAGA, Takeo
MOMOSE, Yulia
MOMOSE, Kunikazu
MASATOMI, Hiroyuki
TERAOKA, Hiroki
キーワード: Grus japonensis
Hokkaido
Japan
mitochondria
Red-crowned Crane
発行日: Jan-2013
出版者: 日本獣医学会
抄録: The Red-crowned Crane, Grus japonensis, is an endangered species of crane that has two separate breeding populations, one in the Amur River basin (continental population) and the other in eastern or northern Hokkaido, Japan (island population). So far, only two haplotypes (Gj1 and Gj2) have been identified in the mitochondrial D-loop in island population, whereas seven haplotypes have been found in continental population (Gj3-Gj9). We developed a rapid and inexpensive method of extensive genotyping of D-loop haplotypes in Red-crowned Cranes, based on amplification refractory mutation system (ARMS) PCR assay. Two hundred and three cranes in eastern Hokkaido were studied with this method and supplemental DNA sequencing. Only two haplotypes, Gj1 and Gj2, were confirmed in eastern Hokkaido with Gj2 as a major haplotype. Additionally, only Gj2 was identified in twelve feathers from both sexes found in northern Hokkaido. These results suggest scarce genetic diversity in island population of Red-crowned Cranes in Hokkaido, Japan.
URI: http://hdl.handle.net/10659/3844
著作権等: ©2013 The Japanese Society of Veterinary Science
出現コレクション:学術論文(雑誌)

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