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タイトル: ウシ体外成熟培養におけるプロジェステロン添加が胚発生に及ぼす影響
その他のタイトル: Effect of progesterone supplementation of maturation medium on the development of IVM-IVF-IVC bovine embryos
著者: 庄司, 圭佑
ショウジ, ケイスケ
発行日: 11-Jun-2014
抄録: The objective of this study was to investigate whether progesterone (P_4) supplementation to in vitro-maturation (IVM) medium could affect the competence of bovine oocyte to develop into blastocysts in vitro. Cumulus—oocyte complexes (COCs) were collected by aspiration of ovarian follicles (2 to 6 mm in diameter) obtained from a local abattoir. The COCs were matured for 20 h in TCM-199 supplemented with 5% calf serum (CS) and 0.02 AU mL^<-1> FSH, at 38.5℃ in an atmosphere of 5% CO_2 in air. P_4 was added to the IVM medium at 10 h after the start of the culture (1 μg mL^<-1> of P_4: 1 μg group; 5 μg mL^<-1> of P_4: 5 μg group; without P_4: control group). The matured COCs were inseminated with 5 x 10^6 sperms mL^<-1>. After 18 h of gamete co-culture, the presumptive zygotes were cultured in CRlaa containing 5% CS at 38.5℃ in an atmosphere of 5% CO_2, 5% 0_2 and 90% N_2 for 9 days (fertilization, day 0). The rates of MII, cleavage and blastocyst formation were analyzed by the chi-square test. Each set of cell numbers (mean ± SE) was analyzed by the unpaired t-test. The MII rate in the control group was significantly lower (P < 0.05) than that in the 1 μg group. The cleavage rate in the 1 μg group was significantly higher (P < 0.05) than those in the control group and 5 μg group. Further, the blastocyst formation rate in the 1 μg group and 5 μg group were significantly higher (P < 0.05) than that in the control group. However, there was no significant difference in cell numbers among the groups. In conclusion, timely P_4 supplementation in IVM medium give rise to high developing rates in bovine embryo cultured in vitro.
URI: http://hdl.handle.net/10659/3417
学位授与年月日: 2013
学位名: 酪農学
学位授与機関: 酪農学園大学
出現コレクション:学位論文(修士)

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